増液承气汤对热结肠道型气分证大鼠肠道组织结构、血清IL-1β、IL-6及TNF-α含量的影响

doi:10.11843/j.issn.0366-6964.2016.04.024

Abstract

Abstract:

In order to study the effects of fluid-increasing decoction for purgation on intestinal structure，concentration of IL-1β，IL-6 and TNF-α in serum of rat in heat accumulation of intestinal type of Qi-fen syndrome，the experiments were carried out to construct the heat accumulation of intestinal type of Qi-fen syndrome，and the fluid-increasing decoction for purgation was given to the rat in preventability or therapeutically，then body temperature，the intestinal organizational structure，and the concentration of inflammatory cytokines in serum of rat were also be studied.The results showed that the intestinal structure in rat in heat accumulation of intestinal type of Qi-fen syndrome were significantly changed，for example，the intestinal mucosa was dropped，inflammatory cells were infiltrated，IELs were increased，goblet cells (GC) were decreased，and the concentration of IL-1β，IL-6 and TNF-α in serum were increased in significantly.The rat were given with the fluid-increasing decoction for purgation in preventability，which will alleviate the damages to intestine of rats，and the concentration of IL-1β，IL-6 and TNF-α decreased significantly；and the rat were given the decoction in therapeutically，which will decrease the body temperature，keep the intestinal structure and mucosal barrier in normal，and decrease the concentration of IL-1β，IL-6 and TNF-α in serum of rats.Therefore，fluid-increasing decoction for purgation which is the classic formula to treat the rats in heat accumulation of intestinal type of Qi-fen syndrome has the preventability and therapeutically effects through decreasing the body temperature，maintaining the intestinal structure in normal，decreasing the IEL’ number and increasing the GC’ number，and then inhibition the secretion of inflammation cytokines.

CLC Number:

S853.9

WANG Zi-li，WANG Xiao-qian，ZHANG Zhu，TU Jun-ping，JIANG Hui-hui，BIE Ying-tang，ZHANG Jia-hua. Effects of Fluid-increasing Decoction for Purgation on Intestinal Structure，Concentration of IL-1β，IL-6 and TNF-α in serum of Rat in Heat Accumulation of Intestinal Type of Qi-fen Syndrome[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 828-835.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2016.04.024

https://www.xmsyxb.com/EN/Y2016/V47/I4/828

References

［1］刘钟杰，许剑琴.中兽医学［M］.3版.北京：中国农业出版社，2002：112-113.
LIU Z J，XU J Q.Traditional Chinese veterinary medicine［M］.3ed.Beijing：China Agriculture Press，2002：112-113.(in Chinese)
［2］尹艳芬，周晋华.增液承气汤临床研究进展［J］.中国中医药现代远程教育，2014，12(16)：163-164.
YIN Y F，ZHOU J H.Research progress of clinical application of Zengye Chengqi decoction［J］.Chinese Medicine Modern Distance Education of China，2014，12(16)：163-164.(in Chinese)
［3］任志兵，温娟，李庆亮，等.增液承气汤治疗5-HT₃受体拮抗剂所致便秘的临床研究［J］.中医临床研究，2014，6(27)：23-25.
REN Z B，WEN J，LI Q L，et al.Clinical study of Zengye Chengqi decoction for the treatment of constipation caused by 5-HT₃ receptor antagonist［J］.Clinical Journal of Chinese Medicine，2014，6(27)：23-25.(in Chinese)
［4］王自力，贺庭祯，张翥，等.大鼠热结肠道型气分证模型的构建及肠道结构、IEL和GC数量的变化研究［J］.畜牧兽医学报，2014，45(4)：672-678.
WANG Z L，HE T Z，ZHANG Z，et al.Study on construction of heat accumulation of intestinal type of Qi-fen syndrome and changes of intestinal structure and number of IEL，GC in rat［J］.Acta Veterinaria et Zootechnica Sinica， 2014，45(4)：672-678.(in Chinese)
［5］张西波，崔乃强，袁红霞，等.阳明腑实证患者内毒素及炎症介质的变化与肠源性内毒素血症的相关性研究［J］.天津中医药，2007，24(3)：187-189.
ZHANG X B，CUI N Q，YUAN H X，et al.Correlation between changes of endotoxin，inflammatory mediators and intestinal endotoxemia in patients with excess syndrome of Yang Ming Fu-organ［J］.Tianjin Journal of Traditional Chinese Medicine，2007，24(3)：187-189.(in Chinese)
［6］周璇，程方平.清热化湿方对温病湿热证模型大鼠血清TNF-α、IL-1β、SIgA的影响［J］.辽宁中医药大学学报，2007，9(2)：142-143.
ZHOU X，CHENG F P.Effects of Qingre Huashi capsule on TNF-α、IL-1β、SIgA concentration in serum dampness-heat model rats［J］.Journal of Liaoning University of Traditional Chinese Medicine，2007，9(2)：142-143.(in Chinese)
［7］MORICHI S，YAMANAKA G，ISHIDA Y，et al.Brain-derived neurotrophic factor and interleukin-6 levels in the serum and cerebrospinal fluid of children with viral infection-induced encephalopathy［J］.Neurochem Res，2014，39(11)：2143-2149.
［8］GÓMEZ C D，BUIJS R M，SITGES M.The anti-seizure drugs vinpocetine and carbamazepine，but not valproic acid，reduce inflammatory IL-1β and TNF-α expression in rat hippocampus［J］. J Neurochem，2014，130(6)：770-779.
［9］HARDEN L M，DU PLESSIS I，POOLE S，et al.Interleukin (IL)-6 and IL-1 beta act synergistically within the brain to induce sickness behavior and fever in rats［J］.Brain Behav Immun，2008，22(6)：838-849.
［10］石玉祥，闫金坤，王雪敏.枸杞多糖对小鼠肠道上皮内淋巴细胞和杯状细胞数量、分布及对IL-2水平影响［J］.食品科学，2011，32(13)：318-320.
SHI Y X，YAN J K，WANG X M.Effect of Chinese wolfberry (Lycium barbarum) polysaccharides on number and distribution of intraepithelial lymphocytes and goblet cells and IL-2 expression in mice［J］. Food Science，2011，32(13)：318-320.(in Chinese)
［11］于征淼，伍军军，赵光峰，等.脂多糖注射法制作兔温病实验模型研究［J］.中华中医药学刊，2010，28(2)：286-288.
YU Z M，WU J J，ZHAO G F，et al.Endue rabbit experimental model of epidemic febrile disease by lipopolysaccharide infusion［J］.Chinese Archives of Traditional Chinese Medicine，2010，28(2)：286-288.(in Chinese)
［12］龚成珍.白虎汤治疗内毒素致家兔温病气分证的实验研究［D］.兰州：甘肃农业大学，2011.
GONG C Z.Experimental study on Baihutang treatment for Qifen syndrome in seasonal febrile disease of endotoxin with rabbits［D］.Lanzhou：Gansu Agricultural University，2011.(in Chinese)
［13］XU R J，WANG F，ZHANG S H.Postnatal adaptation of the gastrointestinal tract in neonatal pigs：a possible role of milk-borne growth factors［J］. Livest Prod Sci，2000，66(2)：95-107.
［14］刘莲莲.仔兔大肠杆菌病小肠黏膜免疫变化及治疗效果观察［D］.泰安：山东农业大学，2012.
LIU L L.Changes of small intestinal mucosal immune of rabbit colibacillosis and comparison of treatment efficiency［D］.Tai’an：Shandong Agricultural University，2012.(in Chinese)
［15］巫全胜，钱宁，吴曙光.药物依赖性便秘大鼠大肠杯状细胞及5-HT变化的实验研究［J］.实验动物科学，2008，25(3)：11-13.
WU Q S，QIAN N，WU S G.An experimental study of the morphological changes of goblet cells and 5-HT immunoreactive cells of intestine of drug-dependent constipation in rats［J］.Laboratory Animal Science，2008，25(3)：11-13.(in Chinese)
［16］徐江红.增液承气汤对家兔排便作用的实验研究［D］.石家庄：河北医科大学，2011.
XU J H.The experimental study of Zengye Chengqi decoction on defecation function in rabbits［D］.Shijiazhuang：Hebei Medical University，2011.(in Chinese)
［17］王文珊，傅冷西，叶君健.TNF-α信号传导通路的研究进展［J］.福建医科大学学报，2005，39(Suppl)：27-31.
WANG W S，FU L X，YE J J.Recent advance of TNF-α signal transduction pathway research［J］.Journal of Fujian Medical University，2005，39(Suppl)：27-31.(in Chinese)
［18］温如果.白虎汤对内毒素性发热家兔解热机制的研究［D］.沈阳：辽宁中医药大学，2009.
WEN R G.The mechanism of Bai-Hu-Tang (BHT) decoction on fever induced by endotoxin(ET)［D］.Shenyang：Liaoning University of Chinese Medicine，2009.(in Chinese)
［19］吴永丰，谢久彬，郭妮.黄连、牡丹皮配伍大承气汤对肠热腑实证大鼠IL-6和NO含量的影响［J］.河南中医，2011，31(9)：988-989.
WU Y F，XIE J B，GUO N.Effects of berberine and cortex moutan compatible with Dachengqi decoction on IL-6 and NO of rats with intestine-heat and Fu-organ excess syndrome［J］.Henan Traditional Chinese Medicine，2011，31(9)：988-989.(in Chinese)
［20］司惠丽，刘春蕾，王世军，等.大黄水煎液对实热证模型大鼠血浆中IL-1β、IL-6、TNF-α水平的影响［J］.山东中医药大学学报，2012，36(1)：58-59.
SI H L，LIU C L，WANG S J，et al.Effects of rheum officinale water extract on IL-1β、IL-6、TNF-α levels in serum of rat in Damp heat syndrome model［J］.Journal of Shandong University of TCM，2012，36(1)：58-59.(in Chinese)

[1]	LEI Yaping, ZHOU Shiwei, TENG Yumeng, NONG Bidan, PANG Xiaomin, ZHANG Yi, LIU Xiaoli, WANG Yulian, HU Xueying. Effect Evaluation of Maxing Shigan Oral Liquid in the Treatment of Broiler Chicken Infected with Mycoplasma gallisepticum [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5264-5274.
[2]	CHANG Weichen, LI Shuaiqi, LI Yan, YAN Wei, ZHANG Hongying, WANG Yanbin, YANG Mingfan, ZHANG Angke. Effect of Pulsatilla Powder Prescription Decoction Ferments on Intestinal Barrier Function of Piglets Infected with Porcine Epidemic Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4403-4410.
[3]	ZHOU Weiwei, WANG Xuefeng, ZHANG Mengjie, YANG Juan, SUN Yuelong, ZHANG Zufeng, ZHANG Yuxin, DOU Jiahong, WANG Ziying, DAI Xiaofeng, LI Xiumei. Analysing the Mechanism of Sihuang Zhili Granule in the Treatment of Piglet Diarrhea Based on Biological Network Function Modules and Compatibility Rules [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3031-3043.
[4]	REN Man, LIU Xin, TANG Yulin, ZHANG Ruixue, QIN Junjie, ZHU Hao, GUO Yansheng. Regulation of Guiqiyimu Compound Preparation on Rumen Microbes and Short-chain Fatty Acids in Postpartum Dairy Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4461-4469.
[5]	GUO Dawei, HOU Silu, CHI Yujia, YU Feike, YU Xiaohan, DENG Qian, XIAO Chuanming, LIU Xiaoye, DONG Hong. Clinical Research of Qiying Decoction and Zigan Decoction on Promoting Reproductive Performance of Sows and Growth Performance of Weaned Piglets [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1994-2004.
[6]	HE Shangwen, WANG Yueming, ZHANG Hui, HOU Silu, LIU Xiaoye, DONG Hong. Pulsatilla Decoction Alleviates LPS-induced Inflammation of Microvascular Endothelial Cells by Inhibiting the TLR4-ERK1/2 Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2317-2325.
[7]	LIU Xiaoxi, MA Yunfei, LI Huanrong, LIU Fenghua, BAO Minglong, ZHANG Jidong, LIN Hongying, XU Jianqin. Effect of Jiawei Gegen Qinlian Decoction on the Regulation of Intestine Damage and Repair in the Piglets of Damp-heat Diarrhea [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 246-255.
[8]	LUO Yichen, YANG Qingchang, LIN Chunfa, YE Ruiling, SHANG Yangfei, HU Yu, LIU Juan. The Effect of Shenfukang Granule on Content of Inflammatory Cytokines and the Signaling Pathway of IL-1R/NF-κB Induced by the Artificial Pathological Model of Chicken Kidney Swelling [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2886-2894.
[9]	LIN Chunfa, LUO Yichen, WANG Zhongqing, FENG Jingbo, LIU Juan. The Clinical Efficacy of BQJGHJ (a Traditional Chinese Medicine Compound Pharmaceutics) in the Treatment of Chickens Artificially Infected with Infectious Bronchitis Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(2): 382-391.
[10]	YAO Wanling, WEN Yanqiao, HUA Yongli, JI Peng, WEI Yanming. Effects of Yujin Powder on SIgA and Inflammatory Cytokines in Ileal and Colonic Mucosa of Large Intestine Dampness-heat Syndrome Rat [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(10): 2147-2155.
[11]	LI Jian, CHEN Yaoxing, CHEN Funing, WANG Zixu, CAO Jing, DONG Yulan, LIU Yang, WANG Xingde, WANG Yang. Protective Effect of Shugan Yiyang Capsule against Quinestrol-induced Testicular Disturbances and Oxidative Stress in Male Rats [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 422-430.
[12]	YAN Zhi-qiang, ZHENG Hua, ZHU Mai-xun, FU Wen-gui, ZHANG Yi-fan, ZHAI Shao-qin. Effect of Nühuang Granules on Spleen Histopathology and Cell Cycle of Immunosuppressive Mice [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2771-2776.
[13]	WEN Yan-qiao, YAO Wan-ling, YANG Chao-xue, MA Qi, ZHANG Xiao-song, JI Peng, HUA Yong-li, WEI Yan-ming. Effects of Yujin Powder on Gastrointestinal Hormone in Serum and Intestinal Tissue of Large Intestine Dampness-heat Syndrome Rat Model [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1140-1149.
[14]	WANG Ming-ming，YANG Shu，DONG Hong，MU Xiang. Pulsatillae Decoction Improves the Bactericidal Capacity of Neutrophils by Protection of Integrity of Microvascular Endothelial Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 836-843.
[15]	LI Xin，LI Zhong-gai，ZHANG Xiao-yi，ZHOU Song-gui，WANG Yu-kun，FENG Jiang，YI Qiong，WANG Lu. Effects of Jin-Ying-Tang on Correlated Molecules of Toll-like Receptor 4 Signaling of LPS-Induced Mouse Mammary Epithelial Cells in vitro [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 609-614.

Effects of Fluid-increasing Decoction for Purgation on Intestinal Structure，Concentration of IL-1β，IL-6 and TNF-α in serum of Rat in Heat Accumulation of Intestinal Type of Qi-fen Syndrome

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments